摘要：A simple, rapid, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of β-sitosterol in the pharmaceutical dosage form of moist exposed burn ointment (MEBO). This method involved an effective sample procedure for extraction of β-sitosterol from MEBO using an alkali saponification agent composed of 0.8?N ethanolic NaOH and diethyl ether. The chromatographic separation was achieved on a C18 column (50?×?3.0?mm, 2.5?μm), using a mobile phase composed of methanol and acetonitrile (70:30 v/v) pumped in an isocratic mode at a flow rate of 0.7 mL/min. The column temperature was maintained at 40?°C, the injection volume was 10 μL, and the detection wavelength was 203?nm. Employing these conditions, the retention time was found to be 2.10?min. The developed method was validated for its specificity, linearity, accuracy, precision, the limit of detection, the limit of quantification, robustness, and solution stability based on International Council for Harmonisation (ICH) guidelines Q2 (R1). Our proposed method demonstrated superior performance compared to other reported methods. It exhibited a linearity range of 30 to 500?μg/mL and improved detectability with a limit of detection (LOD) of 4.65?μg/mL, highlighting its high sensitivity. Additionally, the separation was achieved in a remarkably short analysis time of just 2.1?min, which not only enhanced throughput but also significantly minimized waste and solvent consumption, thereby making it a more sustainable and effective alternative for β-sitosterol extraction. Moreover, in the light of green and white analytical chemistry, a comprehensive ecological and sustainable tri-color coded assessment protocol was established. The proposed method has been successfully applied to quantify β-sitosterol in commercial products (MEBO?, Avomeb? and BISTROl?) demonstrating its suitability for routine quality control analysis of β-sitosterol in pharmaceutical ointment dosage forms.

摘要：Safe cutaneous antisepsis in neonatal intensive care unit is imperative. Chlorhexidine-based solution is commonly used for skin preparation prior to the placement of intravascular devices. However, in premature infants, its usage has not been standardized. We present a case of chemical burns secondary to the use of chlorhexidine-alcohol skin preparation solution in an extremely low birth weight infant.

摘要：BACKGROUND? Postsurgical scars are of significant concern to patients and surgeons. Many studies have focused on different treatment options for established surgical scars. The prevention and minimization of such aberrant healing responses is more likely to yield better outcomes and require less time and expense. OBJECTIVE AND METHOD? A review of the literature on various forms of prophylactic treatments intended to prevent or minimize the development of prominent postsurgical scars was performed using the Pubmed database over a period from 1987 to 2010. Search terms included “scar prevention,” “scar minimization,” “post-surgical scar management,” and “surgical scars.” RESULTS? Various over-the-counter topical products commonly used by patients have failed to demonstrate any significant benefits in improving final scar outcomes. Numerous interventions performed around the time of surgery, including botulinum toxin, lasers, and intradermal injectable products, have shown effectiveness in minimizing eventual scar appearance. CONCLUSIONS? Patient education on proper wound care is a simple method of improving the cosmetic appearance of surgical scars. At the other end of the spectrum, our knowledge of the complex mechanisms of wound healing has allowed for the development of new, effective treatment modalities, including lasers, botulinum toxin, cytokines, and stem cells.

摘要：目的 分析将CO_2点阵激光和湿润烧伤膏用于创伤性瘢痕治疗中的效果。方法 从2021年3月-2022年3月的创伤性瘢痕患者中随机抽取72例，双盲法均分为A组（CO_2点阵激光）和B组（CO_2点阵激光+湿润烧伤膏），对比两组疗效。结果 B组治疗有效率、不良反应发生率为97.22%、5.56%较A组的80.56%、25.00%更优（X25.062、5.257,p0.024、0.021）；B组治疗后治疗效果指标[瘢痕评分（4.41±2.03）分、疼痛评分（2.36±0.59）分、ECCA权重评分（21.15±5.63）分]优于A组的[瘢痕评分（6.59±1.83）分、疼痛评分（3.68±0.72）分、ECCA权重评分（26.84±5.95）分]（t4.785、8.508、4.167，p均0.000）；B组瘢痕指标[疼痛持续时间（2.31±0.67）h、脱痂时间（5.41±1.03）h、疮面愈合时间（8.23±2.14）d]优于A组的[疼痛持续时间（3.34±0.86）h、脱痂时间（6.49±1.15）h、疮面愈合时间（10.56±2.53）d]（t5.668、4.197、4.218，p均0.000）；B组生活质量评分[躯体功能（88.23±5.31）分、社会功能（88.29±5.26）分、生理功能（88.41±5.67）分、心理功能（88.37±5.34）分]高于A组的[躯体功能（81.12±4.52）分、社会功能（81.68±4.71）分、生理功能（81.39±4.58）分、心理功能（81.32±4.68）分]（t6.117、5.617、5.778、5.957，p均0.000）。结论 将CO_2点阵激光和湿润烧伤膏用于创伤性瘢痕患者治疗中，能缩短愈合时间，提高愈合效果和生活品质。