摘要：This study explored the effects of Moist Exposed Burn Ointment (MEBO) on the healing process of pressure-induced wounds. Using a stage IV pressure ulcer model established in 42 rats, divided equally into a control gel group and an MEBO group, we investigated the efficacy of MEBO through topical application. The control group received Carbomer gel, while the MEBO group was treated with MEBO until complete wound healing. Results showed that MEBO significantly accelerated wound healing compared to the control group. Histological analysis, including hematoxylin and eosin (HE) staining and Masson’s trichrome staining, revealed enhanced epithelialization and collagen deposition in the MEBO group. Furthermore, 16S rRNA sequencing indicated that MEBO reduced microbial diversity at the wound site and reshaped the microbial composition. Notably, The increased abundance of Acinetobacter and Staphylococcus, coupled with a reduction in Pseudomonas, may reflect a shift in the wound microbiome that could be conducive to healing. However, the exact role of these microbial shifts in promoting wound healing requires further investigation, as microbial dynamics in wound environments are complex and context-dependent. These findings suggest that MEBO facilitates wound healing by optimizing the wound microbiome, thereby offering a promising therapeutic approach for managing pressure ulcers.

摘要：研究背景和目的糖尿病创面是糖尿病常见并发症,具有病程长、易复发等特点,给患者带来极大困扰,探寻其病理机制及治疗具有重要意义。巨噬细胞和成纤维细胞是创面中的重要效应细胞。现发现ADAM-10调控细胞粘附、生长、迁移等过程。但ADAM-10在糖尿病创面中的具体机制尚不明确。本研究探究ADAM-10在巨噬细胞、成纤维细胞中的调控作用;及其在糖尿病创面愈合过程中的作用机制;结合药物治疗创面,验证研究结果。研究方法1.临床上筛选慢性难愈合创面,随机分组:湿润暴露疗法/湿润烧伤膏(MM)组、重组牛碱性成纤维细胞生长因子凝胶(rb-b FGF)组。在治疗第3、10天,取肉芽及少量上皮组织,免疫荧光(IF)染色ADAM-10、MMP-12,观察蛋白变化特点。2.对sh ADAM-10 RAW264.7细胞进行蛋白组学分析,筛选差异蛋白,并进行生物信息学分析。3.对RAW264.7和J774a.1细胞,进行sh ADAM-10(sh)或EX ADAM-10(EX)干预,随后给予脂多糖(LPS)或MMP-12抑制剂+LPS(MMP-12inh+LPS)刺激,检测细胞活力、基因转录、蛋白表达等情况。4.对L929成纤维细胞进行EX干预,随后LPS刺激,观察细胞活力、ADAM-10以及其它蛋白表达水平。5.正常、糖尿病大鼠分别制备创面,分组为急性(Acute)组、模型(Model)组、MM组以及rb-b FGF组。观察第3、7、10、14天的创面情况;创面率;病理结构;亚细胞结构;ADAM-10转录水平;蛋白表达及定位情况。结果1.慢性难愈合创面治疗第3天,MM组内ADAM-10、MMP-12表达均低于rb-b FGF组。相较于治疗第3天,第10天ADAM-10表达增加;MMP-12降低;MM增加ADAM-10作用低于rb-b FGF;rb-b FGF降低MMP-12作用明显。2.sh RAW264.7细胞蛋白组学检测结果显示:干扰ADAM-10影响创面愈合相关的多种生物学过程,并导致MMP-12和AKT降低。3.巨噬细胞中(1)对较于CON组,1000ng/ml的LPS作用9 h～24 h增加细胞活力(P<0.05);1.5μmol/L的MMP-12inh作用12 h～36 h对细胞活力无影响(P>0.05)。LPS作用12 h显著降低ADAM-10、增加MMP-12基因转录水平(P<0.01);LPS增加MMP-12、i NOS、T...

摘要：Considering the high consumption of and preference for traditional treatments among people and the lack of enough studies on the effectiveness of these treatments, we aimed to review articles on the use of traditional and complementary medicine, focusing on how they affect healing, debridement, and hypertrophic scars. In this study, we conducted the literature search in international databases PubMed, Google Scholar, Ovid, Scopus, Web of Science, and Cochrane Library, as well as Persian databases Scientific Information Database (SID), Magiran, Iranmedex, and IranDoc. Retrieved hits were reviewed by three authors for screening based on inclusion and exclusion criteria, and the screening process is expressed as Preferred Reporting Items for Systematic Reviews and Meta-Analyses framework flow chart. Twenty-two studies were included. The evaluated outcomes were wound healing in 19, pain control in 5, itching in 4, and scar appearance in 2 studies. All of the studies showed equal or improved effects compared to common treatments. Aloe vera, Centella asiatica, and Arnebia euchroma were the most common plants. We revealed that traditional medicine is beneficial in burn wound treatment. The diversity in ingredients that are used in traditional medicine brings up the need for further controlled prospective studies to evaluate the precise superiority of these treatments compared to standard care.

摘要：Objective : Radiodermatitis is a common sequelae in 95% of patients receiving radiation therapy, which is important to be well managed as it can affect the patient's quality of life as well as the cancer treatment schedule. The aim of this study is to review and summarize the interventions available for the treatment of acute radiodermatitis, including traditional Korean medicine, and to propose treatment algorithms for clinicians. Methods : To collect studies about managements for radiodermatitis, domestic and foreign database were used such as Korean journal of traditional knowledge portal (KTKP), Korean studies information service system (KISS), national discovery or science leaders (NDSL), and oriental medicine advance searching integrated system (OASIS), Pubmed, Google scholar and Scopus. Results : Thirty-two studies were selected. There were nine studies on usual care and dressing, eleven studies on chemical agents, two studies on biological preparations, and ten studies on herbal medicines. Conclusion : Hygienic options and dressings have proved to be useful in the management of radiodermatitis. Chemical agents such as corticosteroid, statin, and topical antibiotic agent have proved to alleviate symptoms and severity, regenerate damaged skin, and prevent secondary