摘要：Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healing by regulating autophagy. To explore the mechanism of moist exposed MEBT/MEBO promoting diabetic wound repair. Eighty male Wistar rats were randomly assigned to control (n = 20) and diabetic group induced by intraperitoneal injection of STZ (n = 60), which were further randomly assigned to MEBO, Kangfuxin and model groups (n = 20 each). All rats underwent full-thickness skin resection in the back. Wound healing was dynamically observed and wound tissues were collected at five time points for pathological examination, autophagosome and the expression of PI3K, Akt and mTOR. The healing time in the control group was the shortest, no statistically significant difference was found between the MEBO and the Kangfuxin group (p = 0.76). The morphology of autophagosomes ranged large to small, which was the most obvious in the MEBO group. The mRNA and protein expression of PI3K, Akt and mTOR in each group reached the peak on Day 5, the levels in the MEBO group were the highest (F = 18.43, 19.97, 15.36, p < 0.05). On Day 11, the expression levels in each group began to decline. In this study, we discussed the molecular mechanism of MEBT/MEBO promoting the repair of diabetic ulcer wounds through autophagy and PI3K-Akt-mTOR signalling pathway, which provides a new way for drug design in the future.

摘要：Background MEBO is an analgesic and antibacterial natural agent. Sesame oil extract plays a positive effect in wound healing that may be attributed to its ability to scavenge free radicals and antioxidant properties. Material and methods In this study, MEBO was topically applied to the site of a traumatic oral ulcer to examine its therapeutic effect on pain relief and wound healing. Patients were randomly assigned into two equal groups: group I (MEBO), group II (control), (n?=?15 patients each). Pain was recorded by using the Visual Analogue Scale (VAS), wound size was measured at days 2, 5, and 10. Cytological analysis was also performed using the superficial epithelial cell and keratinization indices. Results There was a statistically significant difference in VAS scores and wound size between MEBO group and control group (p?≤?0.001). Cytological results of MEBO group showed a highly significant improvement (p?≤?0.00001) in re-epithelization and keratinization indices over time. Conclusion Our results showed that the application of MEBO on traumatic ulcer sites has a significant improvement in wound healing and rapid enhancing effect on the re-epithelialization rate.

摘要：Dental Pulp Stem Cells (DPSCs) represent a new therapeutic alternative for treating superficial burn wounds. This study aimed to evaluate the effectiveness of DPSC-derived secretome on superficial burn wounds in an animal model. The study employed a post-test-only controlled group design using 15 rats with superficial burns. Rats were divided into a negative control group of 0.9% NaCl (K1), a positive control group of moist-exposed burn ointment or MEBO (K2), and a treatment group of DPSC-secretome (K3). The effectiveness of the DPSC-secretome was assessed by wound area percentage and histopathological observations on days 5, 14, and 21. DPSCsecretome significantly reduced the burn wound area (p<0.05). Histology data showed that the DPSC-secretome promoted re-epithelialization, fibroblasts, and collagen formation. Polymorphonuclear cells were also reduced after 21 days, indicating that the DPSCs-secretome has an anti-inflammatory effect. In addition, the DPSC-secretome and MEBO showed equal effects on angiogenesis after 14 days. The DPSC-secretome was more effective than MEBO on superficial dermal burns. This study showed that the DPSC secretome has therapeutic potential for burn wounds.

摘要：The presence of necrotic tissue is one of the major problems that affect healing of burn wounds. The present study was designed to find the effectiveness of collagenase versus moist exposed burn ointment (MEBO) on removal of necrotic tissue of burns. Twenty mice randomly assigned and divided into four groups. For Group 1, burn wounds were treated with collagenase ointment only, Group 2 burn wounds were treated with MEBO, Group 3 burn wounds were treated with white vaseline alone, and Group 4 burn wounds were considered as control and left without treatment. In each group, the time of treatment was considered. The results indicated that the removal time of necrotic tissue and healing process was better in the case of using collagenase than using MEBO for treatment of burns.