摘要："烧伤皮肤再生医疗技术(MEBT/MEBO)"是我国烧伤创疡学科带头人徐荣祥教授于1988年发明的,由湿润暴露疗法(MEBT)和湿润烧伤膏(MEBO)组成。当时的医疗技术名称叫"烧伤湿润暴露疗法",1989年国家卫生部通知推荐在全国推广;1991年,国家卫生部将烧伤湿润暴露疗法技术列入首批向全国推广普及的十项重大医药技术。MEBT/MEBO是一种顺应生命规律的全新的烧伤治疗技术与方法,是通过顺应烧伤发病机制制订医疗思路,研究出的一种从局部治疗到

摘要：Objective: From molecular, cell and systemic clinical treatment three levels, this report revealed the mechanism of wound healing, the basic theory and the importance of clinical practice for the Burn Regenerative Therapy with MEBT/MEBO (BRT & MEBT/MEBO).Method: This report analyzed the importance and clinical curative effect for standardized appliance of BRT & MEBT/MEBO from two key points: 1) Liquefaction of necrotic tissue from burn wound; 2) Skin regenerationin situ. Result: There are three necessary conditions for skin physiologically repair and regeneration of burn wound: 1) The formation of moist physiological environment on burn wound; 2) The material foundation of life regenerative substances and histology for keratin-19 stem cells' regenerationin situ; 3) Standardized procedures and appliance of BRT & MEBT/MEBO, which is the guarantee of burn wound healing physiologically. Conclusion: The theory of “Potential Regenerative Cell" and the technique of “Stem cellin situregeneration" are ...

摘要：Objective: To investigate the mechanism of the effect of Moist Exposed Burn Therapy (MEBT) and Moist Exposed Burn Ointment (MEBO) on the spontaneous repair and healing of superficial third degree burn wound involving fatty layer. Method: A series of skin tissue samples were taken from deep burn wounds of 2 cases. Immunocytochemistry method, biotin-avidin DCS system, and indirect immunofluorescence technique were applied. Mouse anti-human keratin type 19 monoclonal antibody was used to detect regenerative epidermal stem cells in wound tissues. Results: Epidermal regenerative stem cells emerged at 24 hours post burn, and the number of epidermal regenerative stem cell increased on day 4 post burn. On days 7 and 14 post burn, the number of epidermal stem cells increased to the peak level. On days 21 and 28 post burn, it decreased and disappeared gradually as burn wound progressed to healing. Conclusion: MEBT/MEBO has the effect of promoting the activation and proliferation of epidermal regenerative stem cells in ...

摘要：目的:慢性难愈合创面是临床中常见的病种之一,但在治疗上缺乏有效的手段干预,越来越多的研究将慢性难愈合创面的治疗靶向转变为促进创面血管新生,探究MEBT/MEBO在促进血管新生过程的作用机制,为临床推广和应用MEBT/MEBO治疗慢性难愈合创面提供理论依据和参考价值;方法:应用付小兵及沈道修相结合的造模方法,分组为空白组、急性创面组、慢性难愈合创面组、贝复济组、MEBT/MEBO组,采用Image J软件、Western Blot、RT-PCR等方法,从宏观到微观观察PTEN-Akt-VEGF信号通路在各组中的动态变化;结果:MEBT/MEBO能够通过调控PTEN-Akt-VEGF信号通路,促进VEGF表达及其mRNA的转录,促进创面血管新生,提高创面愈合率。结论:(1)血管新生是创面修复的一个重要过程,上调VEGF的表达可加速血管新生,并以此促进创面愈合;(2)MEBT/MEBO在慢性难愈合创面的治疗中是安全、高效的治疗手段,可通过调控PTEN-Akt-VEGF信号通路,促进创面血管新生,达到创面愈合的效果。