摘要：To analyze and evaluate the clinical efficacy of Chinese and Western medical techniques in the treatment of severe diabetic foot ulcers complicated with necrotizing fasciitis of the lower leg and summarize the treatment experience of such patients to identify a new method of limb salvage treatment. A total of 46 patients with severe diabetic foot ulcers and necrotizing fasciitis of the lower leg were treated with such techniques as surgical debridement, bone drilling, open joint fusion, and microskin implantation. Wounds were treated with moisture-exposed burn therapy (a regenerative medical treatment for burns, wounds, and ulcers) and moisture-exposed burn ointment (a traditional Chinese medicine); underlying diseases were also treated effectively. The wound healing time, rate of high amputation, and mortality of these patients were summarized, and the clinical efficacy of such treatments was evaluated. Of the 46 patients enrolled, 38 patients were cured, with a cure rate of 82.61%. The average wound healing time was 130?±?74.37 days. Two patients underwent high amputations, with an amputation rate of 4.35%, and 4 deaths occurred, with a mortality rate of 8.70%. The combination of Chinese and Western medical techniques in the treatment of severe diabetic foot ulcers complicated with necrotizing fasciitis of the lower leg not only effectively saved patients' lives and promoted wound healing but also greatly reduced the rates of high amputation and disability.

摘要：AbstrakBerbagai penelitian ilmiah telah membuktikan bahwa kolagen merupakan biomaterial ideal dalam aktivitas penyembuhan luka. Senyawa bioaktif pada madu dan Aloe vera dapat membantu meningkatkan pembentukan kolagen. Penelitian ini dilakukan untuk membandingkan pengaruh aplikasi topikal madu, gel Aloe vera, dan Moist Exposed Burn Ointment (MEBO) terhadap kepadatan kolagen pada proses penyembuhan luka bakar derajat dua pada tikus. Luka bakar dibuat pada 28 ekor tikus dan dibagi menjadi empat kelompok yang dipilih secara acak serta dilakukan aplikasi topikal harian dengan NaCl, madu, gel Aloe vera, dan MEBO secara berurutan. Biopsi kulit dilakukan pada hari ke-7, kemudian dilakukan pembuatan sediaan histopatologi kulit dan dihitung kepadatan kolagennya. Uji One Way ANOVA menunjukkan kepadatan kolagen berbeda secara signifikan antar masing-masing kelompok (p= 0,009). Uji Post Hoc Bonferroni menunjukkan terdapat perbedaan yang signifikan antara kelompok NaCl dengan madu (p= 0,024) dan NaCl dengan MEBO (p= 0,024). Penelitian ini menunjukkan bahwa aplikasi madu dan MEBO secara topikal pada luka bakar derajat dua dapat meningkatkan pembentukan kolagen, sehingga dapat mempercepat proses penyembuhan luka. Madu, Aloe vera, dan MEBO dapat dijadikan sebagai terapi alternatif dalam penyembuhan luka bakar.?AbstractVarious scientific studies have proven that collagen is an ideal biomaterial in wound healing activities. The bioactive compounds in honey and Aloe vera can help increase collagen formation. This study was conducted to compare the effect of topical application of honey, Aloe vera gel, and MEBO (Moist Exposed Burn Ointment) on collagen density in the healing process of second-degree burns in rats. Burns were made on 28 rats which were further randomly divided into four groups to receive daily topical application of NaCl, Aloe vera, honey, and MEBO respectively. Skin biopsy was carried out on the seventh day, then histopathological preparation of the skin was made and collagen density was calculated. Result of One Way ANOVA test showed that collagen density differed significantly between groups (p= 0.009). The Post Hoc Bonferroni test resulted in significant difference between NaCl with honey group (p= 0.024) and NaCl with MEBO (p= 0.024). This study found that topical application of honey and MEBO to second-degree burns could increase collagen formation, thus accelerating wound healing process. Honey, Aloe vera, and MEBO can be used as alternative therapies for healing burns.

摘要：Secretome Dental Pulp Stem Cells have been proposed as a new alternative for wound burn. The aim of this study is to compare Secretome Dental Pulp Stem Cells and MEBO ointment against several fibroblasts and collagen in the healing process of superficial dermal burns. This study used a post-test only controlled group design by using 15 rats that were given superficial dermal burned wounds. Rats were divided into a negative control group of NaCl 0.9% (K1), a treatment group of MEBO (K2), and a treatment group of DPSCs-secretome (K3). Histopathology was done on the 5th, 14th, and 21st days post-induction wound burn. Network-prepared histopathology by colouring Messon Trichomes. Then, it was observed under a microscope with 200x magnification. Scoring in a way semi-qualitative for assessing fibroblasts and collagen. The total score of fibroblasts in the MEBO group was higher than the DPSCs-secretome group on day 5th. In comparison, the score for increasing the amount of collagen in the DPSCs-secretome group on days 5th and 14th was higher than the MEBO group. DPSCs-secretome has better effectiveness in increasing the amount of collagen but not better in increasing fibroblasts compared to MEBO.

摘要：Purpose: This study intended to analyze the differences in the efficacy of three topical wound care products on wound healing in patients undergoing surgery under the laser wound model. Method: A total of 130 patients in the department of dermatology enrolled for retrospective analysis. These patients were divided into group A (n=43, Zihua Shaoshang Ruangao), group B (n=43, Shengji Yuhong Gao), and group C (n=44, Shirun Shaoshang Gao), respectively, according to the type of wound care product administrated. The efficacy was compared during one month of treatment. Results: There was little difference among groups A, B, and C in VAS score, FGF, EGF, and concentration of substance P (SP) at 1, 3, 5, 7, and 10 days after surgery (P>0.05), and a significant difference in these parameters among different time points was observed for intra-group comparison (P<0.05). There was no significant difference in the symptom scores at 1, 5, 10, 15, 20, 25, and 30 days after surgery among the three groups (P>0.05), while there was statistically significant difference at different time points in the same group (P<0.05). The wound healing rates at 10, 20, and 30 days after surgery were 25.58%, 65.12%, and 95.35% in group A, 20.93%, 67.44%, and 100.00% in group B and 25.00%, 59.09%, and 97.73% in group C respectively (P>0.05). The patients’ satisfaction rate towards the appearance was 95.35% in group A, 97.67% in group B, and 97.73% in group C (P>0.05). Conclusion: The three kinds of wound care products, namely Zihua Shaoshang Ruangao, Shengji Yuhong Gao and Shirun Shaoshang Gao, exhibited good efficacy on the wound care of patients after dermatologic surgery. The wounds could be improved quickly, and patients were highly satisfied with the new appearance of the wound. Clinically, wound care products can be selected according to the stock of products in the hospital and patients’ preferences.