摘要：干细胞是动物体内一种独特的基本细胞类型 ,当分化时 ,有些可发展成为特殊类型的细胞、组织和器官 ,另一些仍维持干细胞 ,准备替代机体损伤和衰老的细胞。它们本身能自我复制 ,以供机体终生组织更新的需要。干细胞的培育研究为人类治疗破坏性和消耗性疾病带来了新的希望。国外学者着重于干细胞的离体培育研究 ,而我国徐荣祥运用整体性原则构建了成年皮肤干细胞生长发育的微环境 ,已原位培植成功 ,使烧伤创面达到再生愈合。现就有关干细胞的研究进展作如下阐述

摘要：Objective: From molecular, cell and systemic clinical treatment three levels, this report revealed the mechanism of wound healing, the basic theory and the importance of clinical practice for the Burn Regenerative Therapy with MEBT/MEBO (BRT & MEBT/MEBO).Method: This report analyzed the importance and clinical curative effect for standardized appliance of BRT & MEBT/MEBO from two key points: 1) Liquefaction of necrotic tissue from burn wound; 2) Skin regenerationin situ. Result: There are three necessary conditions for skin physiologically repair and regeneration of burn wound: 1) The formation of moist physiological environment on burn wound; 2) The material foundation of life regenerative substances and histology for keratin-19 stem cells' regenerationin situ; 3) Standardized procedures and appliance of BRT & MEBT/MEBO, which is the guarantee of burn wound healing physiologically. Conclusion: The theory of “Potential Regenerative Cell" and the technique of “Stem cellin situregeneration" are ...

摘要：Objective: To investigate the mechanism of the effect of Moist Exposed Burn Therapy (MEBT) and Moist Exposed Burn Ointment (MEBO) on the spontaneous repair and healing of superficial third degree burn wound involving fatty layer. Method: A series of skin tissue samples were taken from deep burn wounds of 2 cases. Immunocytochemistry method, biotin-avidin DCS system, and indirect immunofluorescence technique were applied. Mouse anti-human keratin type 19 monoclonal antibody was used to detect regenerative epidermal stem cells in wound tissues. Results: Epidermal regenerative stem cells emerged at 24 hours post burn, and the number of epidermal regenerative stem cell increased on day 4 post burn. On days 7 and 14 post burn, the number of epidermal stem cells increased to the peak level. On days 21 and 28 post burn, it decreased and disappeared gradually as burn wound progressed to healing. Conclusion: MEBT/MEBO has the effect of promoting the activation and proliferation of epidermal regenerative stem cells in ...

摘要：<正> In this report, we mainly covered the following aspects of "Tissue organ regeneration and replication in situ": 1) Procedures of tissue organ regeneration and replication and replication in clinical practice; 2) The discover and existence of Potential Regenerative Cell (PRC); 3) The proliferation, differentiation and regeneration law of Potential Regenerative Cells; 4) Study procedure on tissue organ regeneration and replication from PRCs in vitro based on the model of full skin organ regeneration in situ after extensive deep burns; 5) With the well-established model of tissue organ regeneration and replication and replication in vitro, set up the method and technology of searching life regenerative substances required in tissue organ regeneration and replication in situ.In this study, first, the whole human body is divided into 206 function units, which are the "Tissue Organ" in regeneration study. Then the histology foundation of tissue organ regeneration and replication in situ is set up. In order to