摘要：目的:探讨创愈膏在对大鼠溃疡创面愈合不同时间点肉芽组织细胞中转化生长因子(TGF-β1)的含量表达,为进一步验证临床疗效提供实验依据。方法:60只SD大鼠复制成背部感染溃疡创面模型,并随机平均分为创愈膏组、湿润烧伤膏组和生理盐水组,分别外敷创愈膏、湿润烧伤膏和生理盐水纱条换药,并于第3天、第7天、第14天、第21天取创面肉芽组织,用免疫组织化学(SABC)法测量TGF-β1,以阳性细胞数反应其含量变化。结果:第3天、第7天、第14天、第21天,创愈膏组、湿润烧伤膏组肉芽组织TGF-β1均高于生理盐水组(P<0.01);第7天时创愈膏组、湿润烧伤膏组TGF-β1均达到峰值,在第14天时仍保持高水平,二者无显著性差异(P>0.05);而生理盐水组在同一时间点TGF-β1也达到高峰,但明显低于前两组。各时间点创愈膏组高于湿润烧伤膏组,但两组比较,无显著性差异(P>0.05)。结论:创愈膏能增加大鼠溃疡创面肉芽组织细胞中TGF-β1的含量,从而促进创面愈合。

摘要：目的探讨创愈膏在对大鼠溃疡创面愈合不同时间点肉芽组织细胞中转化生长因子(TGF-β1)的含量表达,为进一步验证临床疗效提供实验依据。方法以造成SD大鼠背部感染溃疡创面为模型,分别外敷创愈膏、湿润烧伤膏和生理盐水纱条换药,并于第3、7、14、21天取创面肉芽组织,用免疫组化(SABC)法测量TGF-β1,以阳性细胞数反应其含量变化。结果第3、7、14、21天,创愈膏组、湿润烧伤膏组肉芽组织TGF-β1均高于生理盐水组(P<0.01),且创愈膏组、湿润烧伤膏组第7天时TGF-β1均达到峰值,在第14天时仍保持高水平,二者无显著性差异(P>0.05)。而生理盐水组在同一时间点TGF-β1也达到高峰,但明显低于前两组。各时间点创愈膏组高于湿润烧伤膏组,但两组比较无显著性差异(P>0.05)。结论创愈膏能增加大鼠溃疡创面肉芽组织细胞中TGF-β1的含量,从而促进创面愈合。

摘要：目的观察血浆冷沉淀治疗家兔感染性创面修复的效果,阐述血浆冷沉淀促进家兔感染性创面修复的作用机制。方法将健康的新西兰成年家兔12只,按照改良付小兵全层皮肤缺损法复制创伤感染性动物体表溃疡模型,分别予冷沉淀、康复新液、湿润烧伤膏和凡士林纱布换药,测定治疗后第3、7、12、17天创面微血管密度（MVD）,创面愈合率和新生上皮覆盖率,创面转化生长因子β1（TGF-β1）表达情况。结果各组治疗第3天MVD均呈上升趋势,第3、7天冷沉淀组MVD与其他3组比较,差异有统计学意义（P<0.05）,第12天后新生血管逐渐减少;冷沉淀组创面愈合率、新生上皮覆盖率与康复新液组、凡士林组比较,差异有统计学意义（P<0.05）,冷沉淀组创面完全愈合时间与其他3组比较,差异有统计学意义（H=11.169,P=0.011）,冷沉淀组缩短;冷沉淀组治疗早期TGF-β1表达与其他3组比较差异有统计学意义（P<0.05）,并在整个修复过程中维持高表达。结论冷沉淀可能通过上调创面TGF-β1表达、加速创面MVD,达到提高创面新生上皮覆盖率,促进创面快速愈合的目的。

摘要：Ethnopharmacological relevance The diabetic wound is one of the common chronic complications of diabetes, which seriously affects patients’ quality of life and even causes disability and death. Traditional Chinese medicine (TCM) is a unique and precious resource in China, which has a good curative effect and safety. At present, it has been found that Chinese herbal compounds and effective active ingredients can effectively promote diabetic wound healing, and its mechanism needs to be further studied. Signaling pathways are involved in the pathogenesis and progression of diabetic wounds, which is one of the main targets for the pathologic mechanism of diabetic wounds and the pharmacological research of therapeutic drugs. Aim of the review This study has been carried out to reveal the classical signaling pathways and potential targets by the action of TCM on diabetic wound healing and provides evidence for its clinical efficacy. Materials and methods “diabetic wound”, “diabetic foot ulcer”, “traditional Chinese medicine”, “natural plant” and “medicinal plant”, were selected as the main keywords, and various online search engines, such as PubMed, Web of Science, CNKI and other publication resources, were used for searching literature. Results The results showed that TCM could regulate the signaling pathways to promote diabetic wound healing, such as Wnt, Nrf2/ARE, MAPK, PI3K/Akt, NF-κB, Notch, TGF-β/Smad, HIF-1α/VEGF, which maintaining inflammatory interaction balance, inhibiting oxidative stress and regulating abnormal glucose metabolism. Conclusion The effect of TCM on diabetic wound healing was reflected in multiple levels and multiple pathways. It is envisaged to carry out further research from precision-targeted therapy, provide ideas for screening the core target of TCM in treating diabetic wounds and create modern innovative drugs based on this target.