摘要：目的 通过网络药理学浅究湿润烧伤膏（MEBO）治疗糖尿病足（DF）的作用机制。方法 通过中药系统药理学分析平台（TCMSP）数据库获取MEBO的活性化学成分及有效成分靶点信息，将获得靶点通过UniProt数据库统一基因名称；通过人类基因综合数据库（GeneCards）查询DF的相关靶标基因，利用Venny2.1将MEBO药物活性成分靶点与DF靶点相映射，获得潜在作用靶点。将交集靶点导入STRING网站进行分析，并使用Cytoscape3.10.0构建蛋白质-蛋白质相互作用（PPI）网络图，选出关键靶点基因。将交集靶点导入Metascape进行GO和KEGG通路富集分析，并通过Cytoscape软件构建“药物-成分-靶点”网络图。结果MEBO治疗DF的主要化学成分是小檗碱、汉黄芩素、黄麻苷A、黄连碱、黄柏酮等；关键作用靶点为表皮生长因子受体（EGFR）、一氧化氮合酶3(NOS3)、蛋白激酶B1(AKT1)、白细胞介素-1B(IL-1B)、白细胞介素-6(IL-6)、肿瘤坏死因子（TNF）等；关键作用通路为AGE-RAGE、HIF-1、VEGF、NF-κB等信号通路。结论 本研究初步揭示了MEBO的有效成分可以从多靶点、多通路治疗DF，发挥着改善微环境、抑制创面感染及促进组织修复再生等作用。

摘要：目的观察原位再生医疗技术对Ⅲ、Ⅳ期压疮创面面积及血管内皮生长因子（Vascular Endothelial Growth Factor,VEGF）表达的影响,探讨其促进压疮创面愈合的可能机制。方法将2015年1月-2016年3月广西壮族自治区江滨医院收治的45例Ⅲ、Ⅳ期压疮患者按随机数表法随机分为治疗组（24例）与对照组（21例）,治疗组患者创面在彻底清创后采用原位再生医疗技术治疗,对照组患者创面在彻底清创后采用凡士林油纱换药治疗。采用免疫组织化学方法检测治疗第7天两组患者压疮创面组织内VEGF的表达情况;采用透明板描绘加坐标纸法测量治疗前及治疗第30天两组患者压疮创面面积,对比创面面积变化情况。结果治疗第7天,治疗组压疮创面组织内VEGF的MOD值为0.40±0.12,明显高于对照组的0.31±0.11,t=2.18,P<0.05,差异具有统计学意义;治疗第30天,治疗组压疮创面平均面积由治疗前的（105.0±32.8）cm2缩小为（46.4±15.0）cm2,对照组压疮创面平均面积由治疗前的（101.2±30.4）cm2缩小为（65.5±20.5）cm2,两组对比,t=3.30,P<0.05,差异具有统计学意义。结论原位再生医疗技术可通过诱导VEGF的表达促进Ⅲ、Ⅳ期压疮创面愈合。

摘要：目的:探讨皮肤原位再生医疗技术(Moist exposed burn therapy/moist exposed burn ointment,MEBT/MEBO)对四肢创伤创面皮肤屏障功能及血管生成的影响。方法:选取2017年9月-2019年9月笔者医院110例四肢创伤患者,随机数字表法分组,各55例。对照组施行负压封闭引流技术(Vacuum sealing drainage,VSD),实验组施行MEBT/MEBO。观察两组治疗效果、创面愈合时间、疼痛程度、住院时间及并发症情况,对比治疗前、治疗后两周血管生成指标[微血管密度(Microvessel density,MVD)、血管内皮生长因子(Vascular endothelial growth factor,VEGF)、S100钙结合蛋白A9(Serum calcium-binding protein A9,S100A9)]、皮肤屏障功能[经皮水份丢失量(Transepider-malwaterloss,TEWL)、皮肤表面pH值],并于治疗后24周随访瘢痕程度(Vancouver scar scale,VSS)。结果:实验组治疗总有效率90.91%高于对照组的74.55%,差异有统计学意义(P<0.05)。实验组创面愈合时间、住院时间短于对照组,VAS评分低于对照组,差异有统计学意义(P<0.05)。治疗后两周实验组肉芽组织中MVD、VEGF、S100A9水平高于对照组,差异有统计学意义(P<0.05)。治疗后两周两组TEWL低于治疗前,皮肤表面pH值高于治疗前,但组间比较差异无统计学意义(P>0.05)。实验组并发症发生率5.45%低于对照组的18.18%,差异有统计学意义(P<0.05)。治疗后24周实验组VSS评分低于对照组,差异有统计学意义(P<0.05)。结论:MEBT/MEBO应用于四肢创伤创面,有助于促进血管生成,减轻疼痛,缩短创面愈合时间,降低并发症,提高治疗效果。

摘要：Ethnopharmacological relevance The diabetic wound is one of the common chronic complications of diabetes, which seriously affects patients’ quality of life and even causes disability and death. Traditional Chinese medicine (TCM) is a unique and precious resource in China, which has a good curative effect and safety. At present, it has been found that Chinese herbal compounds and effective active ingredients can effectively promote diabetic wound healing, and its mechanism needs to be further studied. Signaling pathways are involved in the pathogenesis and progression of diabetic wounds, which is one of the main targets for the pathologic mechanism of diabetic wounds and the pharmacological research of therapeutic drugs. Aim of the review This study has been carried out to reveal the classical signaling pathways and potential targets by the action of TCM on diabetic wound healing and provides evidence for its clinical efficacy. Materials and methods “diabetic wound”, “diabetic foot ulcer”, “traditional Chinese medicine”, “natural plant” and “medicinal plant”, were selected as the main keywords, and various online search engines, such as PubMed, Web of Science, CNKI and other publication resources, were used for searching literature. Results The results showed that TCM could regulate the signaling pathways to promote diabetic wound healing, such as Wnt, Nrf2/ARE, MAPK, PI3K/Akt, NF-κB, Notch, TGF-β/Smad, HIF-1α/VEGF, which maintaining inflammatory interaction balance, inhibiting oxidative stress and regulating abnormal glucose metabolism. Conclusion The effect of TCM on diabetic wound healing was reflected in multiple levels and multiple pathways. It is envisaged to carry out further research from precision-targeted therapy, provide ideas for screening the core target of TCM in treating diabetic wounds and create modern innovative drugs based on this target.