摘要：Dental Pulp Stem Cells (DPSCs) represent a new therapeutic alternative for treating superficial burn wounds. This study aimed to evaluate the effectiveness of DPSC-derived secretome on superficial burn wounds in an animal model. The study employed a post-test-only controlled group design using 15 rats with superficial burns. Rats were divided into a negative control group of 0.9% NaCl (K1), a positive control group of moist-exposed burn ointment or MEBO (K2), and a treatment group of DPSC-secretome (K3). The effectiveness of the DPSC-secretome was assessed by wound area percentage and histopathological observations on days 5, 14, and 21. DPSCsecretome significantly reduced the burn wound area (p<0.05). Histology data showed that the DPSC-secretome promoted re-epithelialization, fibroblasts, and collagen formation. Polymorphonuclear cells were also reduced after 21 days, indicating that the DPSCs-secretome has an anti-inflammatory effect. In addition, the DPSC-secretome and MEBO showed equal effects on angiogenesis after 14 days. The DPSC-secretome was more effective than MEBO on superficial dermal burns. This study showed that the DPSC secretome has therapeutic potential for burn wounds.
摘要：Purpose: This study intended to analyze the differences in the efficacy of three topical wound care products on wound healing in patients undergoing surgery under the laser wound model. Method: A total of 130 patients in the department of dermatology enrolled for retrospective analysis. These patients were divided into group A (n=43, Zihua Shaoshang Ruangao), group B (n=43, Shengji Yuhong Gao), and group C (n=44, Shirun Shaoshang Gao), respectively, according to the type of wound care product administrated. The efficacy was compared during one month of treatment. Results: There was little difference among groups A, B, and C in VAS score, FGF, EGF, and concentration of substance P (SP) at 1, 3, 5, 7, and 10 days after surgery (P>0.05), and a significant difference in these parameters among different time points was observed for intra-group comparison (P<0.05). There was no significant difference in the symptom scores at 1, 5, 10, 15, 20, 25, and 30 days after surgery among the three groups (P>0.05), while there was statistically significant difference at different time points in the same group (P<0.05). The wound healing rates at 10, 20, and 30 days after surgery were 25.58%, 65.12%, and 95.35% in group A, 20.93%, 67.44%, and 100.00% in group B and 25.00%, 59.09%, and 97.73% in group C respectively (P>0.05). The patients’ satisfaction rate towards the appearance was 95.35% in group A, 97.67% in group B, and 97.73% in group C (P>0.05). Conclusion: The three kinds of wound care products, namely Zihua Shaoshang Ruangao, Shengji Yuhong Gao and Shirun Shaoshang Gao, exhibited good efficacy on the wound care of patients after dermatologic surgery. The wounds could be improved quickly, and patients were highly satisfied with the new appearance of the wound. Clinically, wound care products can be selected according to the stock of products in the hospital and patients’ preferences.
摘要：Skin wounds represent an enormous clinical and financial problem in equine. The present study evaluated the effect of Panthenol gel and Mebo ointment on metacarpal wounds healing in donkeys. This experimental study was carried out on eighteen adult donkeys divided into three groups each of six donkeys. All animals were exposed to creation of 2?× 2 cm full-thickness skin wound on the dorsal aspect of the metacarpal regions of both forelimbs. All the wounds were cleaned daily with sterile gauze soaked with sterile distilled water. For the Panthenol gel group, 1 finger tip unit (0.5?g) of Panthenol gel was applied once daily over the wound surfaces. For the Mebo ointment group, 1 finger tip unit (0.5?g) of Mebo ointment was applied once daily over the wound surfaces. For control group, the wounds were cleaned with sterile gauze soaked with sterile distilled water without application of any treatment. The wound healing process was compared by gross observation, geometrical assessments of percentage wound contraction, percentage wound epithelialization, percentage wound healing, and histopathological examination. The wound contraction, wound epithelialization, and wound healing percentages in the Panthenol gel group were significantly higher than other groups and by the end of seventh week, all animals’ wounds in the Panthenol gel group had complete healing. Mebo ointment group had no a significant effect on wound epithelialization and wound healing percentages. In conclusion, the study recommended the use of Panthenol gel as an effective and low-cost wound healing promoting agent for full-thickness skin wounds in donkeys.